CODONW(1)

✨ CODONW — the correspondence analysis of codon usage glow-up ✨

okay bestie, sit down, hydrate, because this is the codonw manual page and it's literally about making sense of codon usage at scale 💖 (the title up top says CODONW(1) and the manual name is codonw, in case you were wondering who the main character is)

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🌸 SYNOPSIS 🌸

codonw [inputfile] [outputfile] [bulkoutfile] [options]

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🦋 DESCRIPTION 🦋

CodonW is a whole package for codon usage analysis, and honestly? It was built to make Multivariate Analysis (MVA) of codon usage so much simpler. The MVA method it uses is correspondence analysis (COA) — which is, no cap, the most popular MVA method for codon usage analysis. 💅

CodonW can generate a COA for:

• codon usage
• relative synonymous codon usage
• amino acid usage

And it also does extra analyses of codon usage, including investigating optimal codons, codon and dinucleotide bias, and/or base composition. it's giving full-service, periodt ✨

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💖 OPTIONS 💖

• -h — Show help message
• -nomenu — Prevent the menu interface being displayed
• -nowarn — Prevent warnings about sequences being displayed
• -silent — Overwrite files silently
• -totals — Concatenate all genes in inputfile
• -machine — Machine readable output
• -human — Human readable output
• -code N — Genetic code as defined under menu 3 option 5
• -f_type N — Fop/CBI codons as defined by menu 3 option 6
• -c_type N — Cai fitness values as defined by menu 3 option 7
• -t (char) — Column separator to be used in output files (comma, tab, space)

🎀 Codon usage indices and Amino acid indices 🎀

• -cai — calculate Codon Adaptation Index (CAI)
• -fop — calculate Frequency of OPtimal codons index (FOP)
• -cbi — calculate Codon Bias Index (CBI)
• -enc — Effective Number of Codons (ENc)
• -gc — G+C content of gene (all 3 codon positions)
• -gcs3 — GC of synonymous codons 3rd positions
• -sil_base — Base composition at synonymous third codon positions
• -L_sym — Number of synonymous codons
• -L_aa — Total number of synonymous and non-synonymous codons
• -all_indices — All the above indices
• -aro — Calculate aromaticity of protein
• -hyd — Calculate hydropathicity of protein
• -cai_file {file} — User input file of CAI values
• -cbi_file {file} — User input file of CBI values
• -fop_file {file} — User input file of Fop values

🌈 Correspondence analysis (COA) options 🌈

• -coa_cu — COA of codon usage frequencies
• -coa_rscu — COA of Relative Synonymous Codon Usage
• -coa_aa — COA of amino acid usage frequencies
• -coa_expert — Generate detailed (expert) statistics on COA
• -coa_axes N — Select number of axis to record
• -coa_num N — Select number of genes to use to identify optimal codons values, can be whole numbers or a percentage (5 or 10%)

🫶 Bulk output options 🫶 | only one can be selected per analysis

bestie this is a pick one situation, not a buffet 🍓

• -aau — Amino Acid Usage (AAU)
• -raau — Relative Amino Acid Usage (RAAU)
• -cu — Codon Usage (CU) (default)
• -cutab — Tabulation of codon usage
• -cutot — Tabulation of dataset's codon usage
• -rscu — Relative Synonymous Codon Usage (RSCU)
• -fasta — fasta format
• -tidy — fasta format
• -reader — Reader format (codons are separated by spaces)
• -transl — Conceptual translation of DNA to amino acid
• -base — Detailed report of codon G+C composition
• -dinuc — Dinucleotide usage of the three codon pos.
• -noblk — No bulk output to be written to file

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Some of the codonw subtools can be directly accessed using shortcuts prefixed with codonw-. iykyk 😌

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👑 AUTHOR 👑

This manpage was written by Sascha Steinbiss for the Debian distribution and can be used for any other usage of the program. iconic behaviour fr fr 💜

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🧚 SEE ALSO 🧚

codonw-rscu(1), codonw-cu(1), codonw-aau(1), codonw-fop(1), codonw-raau(1), codonw-tidy(1), codonw-reader(1), codonw-cutab(1), codonw-cutot(1), codonw-transl(1), codonw-bases(1), codonw-base3s(1), codonw-dinuc(1), codonw-cai(1), codonw-gc3s(1), codonw-gc(1), codonw-cbi(1), codonw-enc(1)

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and that's the whole page, bestie 🎀 this was codonw version 1.4.4, dated February 2021 — go forth and analyse those codons, you absolute legend 💅✨