ASSEMBLEPAIRS.PY(1)

Okay bestie, welcome to the User Commands manual page for ASSEMBLEPAIRS.PY(1) πŸŽ€βœ¨ yes, this is the one, iykyk πŸ’…

✨ NAME ✨

AssemblePairs.py - Assembles paired-end reads into a single sequence πŸ¦‹ it literally takes your paired-end reads and glues them into one sequence, no cap πŸ’–

✨ DESCRIPTION ✨

Okay so here's the usage line, and it's giving main character energy πŸ‘‘

usage: AssemblePairs.py [--version] [-h]  ...

Same vibe as the NAME, fr fr. It's all about assembling paired-end reads into a single sequence 🧚

πŸŽ€ help

  • --version β€” shows the program's version number and then bounces πŸ‘‹
  • -h, --help β€” shows the help message and exits 🫢

🌸 subcommands

This is where the magic happens ✨ There's an Assembly method section, and these are your options:

  • align β€” assembles pairs by aligning the ends πŸ’…
  • join β€” assembles pairs by concatenating the ends πŸŽ€
  • reference β€” assembles pairs by aligning reads against a reference database 🌈
  • sequential β€” assembles pairs by first attempting de novo assembly, then reference guided assembly. Lowkey the most thorough one πŸ”₯

πŸ“ output files

  • assemble-pass β€” successfully assembled reads, ayyy πŸ¦‹
  • assemble-fail β€” raw reads that failed paired-end assembly πŸ’€

🌈 output annotation fields

  • <user defined> β€” annotation fields you set with the --1f or --2f arguments πŸ“

✨ AUTHOR ✨

Andreas Tille wrote this manpage for the Debian distribution, and it's giving "use it for any other usage of the program too" energy 🫢


AssemblePairs.py version 0.6.0, May 2020. That's the tea, bestie πŸ’œπŸŒˆ