6FT(1)

✨ NAME ✨

Okay bestie, this is the 6FT(1) page from the pftools package, and it's all about 6ft 💖

6ft is a 6-frame translation of a DNA sequence into protein 🧚

🎀 SYNOPSIS 🎀

6ft       [ -h ] [ -r | -s ] [ -W width ] < sequence_library

💅 DESCRIPTION 💅

Okay so 6ft translates all six reading frames of a double-stranded DNA sequence into individual protein sequences 🦋 Stop codons show up as the character O, which is iconic, lol.

The program reads input fasta formatted DNA sequences from standard input 🫶 If your sequences live in a file, redirect the input from that file with the shell's input redirector <, just like in the synopsis section. The sequence_library holds one or several sequences in Pearson/Fasta format ✨

This one is giving genomic or EST sequence database vibes 🌸 You can use it to search those databases with a protein profile. Better results are usually obtained by first converting the protein profile into a frame-search profile, and then searching a 2-frame translation of the DNA sequence database generated with the pftools program 2ft(1). Check out the manual pages of the pftools programs ptof(1) and 2ft(1) for the deets 💜

🌈 OPTIONS 🌈

• -h — show the usage help text 💖
• -r — translate only the reverse (antisense) strand 🔄
  • This option is mutually exclusive with -s.
  • Default: translate both strands
• -s — translate only the sense strand 🌊
  • This option is mutually exclusive with -r.
  • Default: translate both strands
• -W width — set the sequence output width 📏
  • The value of width says how many residues get printed on one line.
  • Type: integer
  • Default: 60

🧚 EXAMPLES 🧚

Go check out the pftools program ptof(1) for those 🙈

🔗 SEE ALSO 🔗

ptof(1), pfsearch(1), pfscan(1) ⭐

👑 AUTHOR 👑

The pftools package was developed by Philipp Bucher 👑 Any comments or suggestions should go to <pftools@sib.swiss> 💌

That's the tea ☕ This was pftools 2.3, from May 2003, 6FT(1) 🎀 Stay iconic, bestie! 💖✨