PRIMER_DESIGNER(1p)

💅 PRIMER_DESIGNER(1p) 💅

okay bestie, welcome to primer_designer.pl — the command-line interface for Bio::PrimerDesigner. yes it's a Perl manpage from the User Contributed Perl Documentation shelf, and yes it's lowkey iconic. sit down, hydrate, let's get into it. 🫶

✨ SYNOPSIS ✨

here's the whole vibe in one line:

./primer_designer.pl [options] [dna_sequence or file]

🎀 Options

• -p|--program — which program you're running. default is "primer3".
• -n|--number — how many primer sets you want back. default is "5".
• -b|--binary — path to the binary executable. default is "/usr/local/bin".
• -m|--method — "local" or "remote". default is "local".
• -u|--url — the URL of the remote primer3/e-PCR system. default is "aceserver.biotech.ubc.ca/cgi-bin/primer_designer.cgi".
• --list-aliases — prints the alias list for primer3 input options and exits. 👋
• --list-params — prints a list of primer3/e-PCR input options and exits. also 👋
• -h|--help — prints help and exits. the classic. 💖

🌸 DESCRIPTION 🌸

so what does this script even do? it tests and demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. that's the whole assignment, and it ate. 💅

the way it works is: you can point it at a local installation of the unix binaries, or you can go remote and do it all over an HTTP/CGI request. two paths, both valid, main character energy either way. ✨

👑 AUTHOR 👑

Copyright (C) 2003-2009 Sheldon McKay <mckays@cshl.edu>, Ken Youens-Clark <kclark@cpan.org>.

🔍 SEE ALSO 🔍

Bio::PrimerDesigner.

📜 LICENSE 📜

This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.

no cap on the license, bestie — read it, it's literally the whole point of the "free software" thing. 🫶

and that's the page, fr fr. 💖 this one shipped as perl v5.32.0, dated 2020-11-16, filed under PRIMER_DESIGNER(1p). mwah. 😘🎀🦋