SHOWALIGN(1e)

🌸 SHOWALIGN(1e) 🌸

okay bestie, welcome to the EMBOSS Manual for Debian page for showalign, and no cap, this one is lowkey iconic.

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✨ NAME ✨

showalign — display a multiple sequence alignment in pretty format 💅

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🦋 SYNOPSIS 🦋

showalign -sequence seqset [-matrix matrix] [-refseq string]
          [-bottom boolean] [-show list] [-order list]
          [-similarcase boolean] [-consensus boolean] -uppercase range
          -number boolean -ruler boolean -width integer -margin integer
          -html boolean -highlight range -plurality float -setcase float
          -identity float -gaps boolean -outfile outfile

showalign -help

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💖 DESCRIPTION 💖

so showalign is a command line program from EMBOSS — that's "the European Molecular Biology Open Software Suite," periodt. it lives in the "Alignment:Multiple,Data retrieval:Sequence data" command group(s). it's giving alignment-glow-up energy fr fr 🌈

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🎀 OPTIONS 🎀

🌷 Input section 🌷

• -sequence seqset — the sequence alignment you wanna display. the whole main character of this operation 💜
• -matrix matrix — this is the scoring matrix file used when comparing sequences. by default it's the file 'EBLOSUM62' (for proteins) or the file 'EDNAFULL' (for nucleic sequences). these files are found in the 'data' directory of the EMBOSS installation. so yeah, it already knows what to pick, you don't gotta stress 🫶

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🌸 Additional section 🌸

• -refseq string — if you give the number in the alignment or the name of a sequence, it will be taken to be the reference sequence. the reference sequence is always shown in full and is the one against which all the other sequences are compared. if this is set to 0 then the consensus sequence will be used as the reference sequence. by default the consensus sequence is used as the reference sequence. so it's the queen bee everyone gets compared to 👑
• -bottom boolean — if this is true then the reference sequence is displayed at the bottom of the alignment instead of the top. Default value: Y
• -show list — Default value: N
• -order list — Default value: I
• -similarcase boolean — if this is set True, then when -show is set to 'Similarities' or 'Non-identities' and a residue is similar but not identical to the reference sequence residue, it will be changed to lower-case. if -show is set to 'All' then non-identical, non-similar residues will be changed to lower-case. if this is False then no change to the case of the residues is made on the basis of their similarity to the reference sequence. Default value: Y
• -consensus boolean — if this is true then the consensus line is displayed. Default value: Y

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🧚 Advanced section 🧚

• -uppercase range — regions to put in uppercase. if this is left blank, then the sequence case is left alone. a set of regions is specified by a set of pairs of positions. the positions are integers. they are separated by any non-digit, non-alpha character. examples of region specifications are:

24-45, 56-78
1:45, 67=99;765..888
1,5,8,10,23,45,57,99

• -number boolean — if this option is true then a line giving the positions in the alignment is displayed every 10 characters above the alignment. Default value: Y
• -ruler boolean — if this option is true then a ruler line marking every 5th and 10th character in the alignment is displayed. Default value: Y
• -width integer — Default value: 60
• -margin integer — this sets the length of the left-hand margin for sequence names. if the margin is set at 0 then no margin and no names are displayed. if the margin is set to a value that is less than the length of a sequence name then the sequence name is displayed truncated to the length of the margin. if the margin is set to -1 then the minimum margin width that will allow all the sequence names to be displayed in full plus a space at the end of the name will automatically be selected. Default value: -1
• -html boolean — Default value: N
• -highlight range — regions to colour if formatting for HTML. if this is left blank, then the sequence is left alone. a set of regions is specified by a set of pairs of positions. the positions are integers. they are followed by any valid HTML font colour. examples of region specifications are:

24-45 blue 56-78 orange 1-100 green 120-156 red

a file of ranges to colour (one range per line) can be specified as '@filename'.

• -plurality float — set a cut-off for the % of positive scoring matches below which there is no consensus. the default plurality is taken as 50% of the total weight of all the sequences in the alignment. Default value: 50.0
• -setcase float — sets the threshold for the scores of the positive matches above which the consensus is in upper-case and below which the consensus is in lower-case. by default this is set to be half of the (weight-adjusted) number of sequences in the alignment. Default value: @( $(sequence.totweight) / 2)
• -identity float — provides the facility of setting the required number of identities at a position for it to give a consensus. therefore, if this is set to 100% only columns of identities contribute to the consensus. Default value: 0.0
• -gaps boolean — if this option is true then gap characters can appear in the consensus. the alternative is 'N' for nucleotide, or 'X' for protein. Default value: Y

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🍓 Output section 🍓

• -outfile outfile

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💀 BUGS 💀

bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031). like, if something's acting up, tell them, don't suffer in silence bestie 😭

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🌈 SEE ALSO 🌈

showalign is fully documented via the tfm(1) system.

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👑 AUTHOR 👑

Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>

• wrote the script used to autogenerate this manual page. slay, honestly ✨

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💅 COPYRIGHT 💅

this manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. it can be redistributed under the same terms as EMBOSS itself.

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and that's the whole vibe — this page is EMBOSS 6.4.0, dated 05/11/2012, and it's filed under SHOWALIGN(1e). go forth and display those alignments, gorgeous 🦋💖