PRIMA(1e)

💖 prima — the primer-picking queen 💖

okay so bestie, welcome to prima(1e), a page from the EMBOSS Manual for Debian. if you've ever needed to pick primers for PCR and DNA amplification and wanted a command-line bestie to do it with you, this is literally her. she's part of the EMBOSS suite ("the European Molecular Biology Open Software Suite" — yes she's fancy) and she hangs out in the "Nucleic:Primers" command group(s). periodt. 👑

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✨ SYNOPSIS ✨

prima -sequence sequence -targetrange toggle -targetstart integer
      -targetend integer -mintmprimer float -maxtmprimer float
      -minplen integer -maxplen integer -minpgccont float
      -maxpgccont float [-primer toggle] -minprimerlen integer
      -maxprimerlen integer -minpmgccont float -maxpmgccont float
      -overlap integer -saltconc float -dnaconc float -list boolean
      -outfile outfile

prima -help

see how -primer is the only one chillin' in square brackets? yeah, she's optional, everyone else is required. main character energy honestly. 💅

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🌸 DESCRIPTION 🌸

so prima is a command line program from EMBOSS ("the European Molecular Biology Open Software Suite"). she lives in the "Nucleic:Primers" command group(s). that's it, that's the vibe. she selects primers for PCR and DNA amplification and she's very good at it, no cap. 🧬✨

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🎀 OPTIONS 🎀

🌈 Input section

• -sequence sequence — bestie, this is your input sequence. give her the goods. 🫶

👑 Required section

🎯 Target options

• -targetrange toggle — default value: N
• -targetstart integer — default value: $(sequence.begin)
• -targetend integer — default value: $(sequence.length)
• -mintmprimer float — default value: 53
• -maxtmprimer float — default value: 58

💜 Additional section

🍓 Product options

• -minplen integer — default value: 100
• -maxplen integer — default value: 300
• -minpgccont float — default value: .40
• -maxpgccont float — default value: .55

🧚 Primer options

• -primer toggle — specify primer properties. default value: Y
• -minprimerlen integer — default value: 18
• -maxprimerlen integer — default value: 22
• -minpmgccont float — default value: .40
• -maxpmgccont float — default value: .55

🔥 Advanced section

• -overlap integer — default value: 50
• -saltconc float — default value: 50
• -dnaconc float — default value: 50
• -list boolean — default value: N

⭐ Output section

• -outfile outfile — where the results go, bestie. 🌟

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💀 BUGS 💀

found a bug? not the vibe, but okay, report it to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or slide directly into the EMBOSS developers' DMs (http://sourceforge.net/tracker/?group_id=93650&atid=605031). they wanna know, fr fr. 🙈

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👀 SEE ALSO 👀

prima is fully documented via the tfm(1) system. go check it out, iykyk. 💖

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🫶 AUTHOR 🫶

Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>

• wrote the script used to autogenerate this manual page. iconic behaviour, honestly. 🎀

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🌟 COPYRIGHT 🌟

this manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. it can be redistributed under the same terms as EMBOSS itself. slay. ✨

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and that's the whole page bestie!! EMBOSS 6.4.0, dated 05/11/2012, and she goes by PRIMA(1e). go pick some primers, you're gonna ate. 💅🌈💖