EPESTFIND(1e)

💖 EPESTFIND(1e) — the EMBOSS Manual for Debian glow-up 💖

okay bestie, sit down, hydrate, and let's talk about epestfind, a whole entire member of the EMBOSS family (you know, the European Molecular Biology Open Software Suite — yes that's the real name, yes it's iconic).

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✨ NAME ✨

epestfind — Finds PEST motifs as potential proteolytic cleavage sites 🦋

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🌸 SYNOPSIS 🌸

like, here's exactly how you call it, no cap:

epestfind -sequence sequence [-mwdata datafile] -window integer
          -order selection [-threshold float] -mono boolean
          -potential boolean -poor boolean -invalid boolean -map boolean
          -outfile outfile -graph xygraph

epestfind -help

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💅 DESCRIPTION 💅

So epestfind is a command line program from EMBOSS ("the European Molecular Biology Open Software Suite"). She's part of the "Protein:Motifs" command group(s), and she's lowkey the moment. Periodt. 👑

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🎀 OPTIONS 🎀

🌈 Input section

• -sequence sequence — Protein sequence USA to be analysed. give her the goods 💖
• -mwdata datafile — Default value: Emolwt.dat

⭐ Required section

• -window integer — Minimal distance between positively charged amino acids. Default value: 10
• -order selection — Name of the output file which holds the results of the analysis. Results may be sorted by length, position and score. Default value: score

🦋 Additional section

• -threshold float — Threshold value to discriminate weak from potential PEST motifs. Valid PEST motifs are discriminated into 'poor' and 'potential' motifs depending on this threshold score. By default, the default value is set to +5.0 based on experimental data. Alterations are not recommended since significance is a matter of biology, not mathematics. Default value: +5.0

ngl bestie, do not go messing with -threshold for fun. the manpage literally says alterations are not recommended, and biology said what it said. 🫶

💜 Advanced section

• -mono boolean — Default value: N
• -potential boolean — Decide whether potential PEST motifs should be printed. Default value: Y
• -poor boolean — Decide whether poor PEST motifs should be printed. Default value: Y
• -invalid boolean — Decide whether invalid PEST motifs should be printed. Default value: N
• -map boolean — Decide whether PEST motifs should be mapped to sequence. Default value: Y

🍓 Output section

• -outfile outfile — Name of file to which results will be written.
• -graph xygraph

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😭 BUGS 😭

Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).

found a bug? not you being the main character who reports it. do it, bestie, they wanna hear from you 💀

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🔥 SEE ALSO 🔥

epestfind is fully documented via the tfm(1) system.

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👑 AUTHOR 👑

Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>

• Wrote the script used to autogenerate this manual page.

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🌸 COPYRIGHT 🌸

This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.

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and that's the whole vibe check, bestie 💖 this lil page was brought to you by EMBOSS 6.4.0, dated 05/11/2012 — fr fr, she's been slaying since way back. go find those PEST motifs, you got this 🧚✨