VECSCREEN(1)

💖 VECSCREEN(1) 💖

okay bestie, so this is a page from the NCBI Tools User's Manual, and it's all about a little tool called vecscreen. stay with me, it's actually so good.

✨ NAME ✨

vecscreen — find nucleic acid segments of possible vector origin 🦠🔍

🌸 SYNOPSIS 🌸

vecscreen [-] [-d str] [-f N] [-i filename] [-o filename]

🦋 DESCRIPTION 🦋

so. vecscreen is a system for quickly identifying segments of a nucleic acid sequence that may be of vector origin. NCBI developed vecscreen to minimize the incidence and impact of vector contamination in public sequence databases. GenBank Annotation Staff use vecscreen to verify that sequences submitted for inclusion in the database are free from contaminating vector sequence. and literally any sequence can be screened for vector contamination using vecscreen. no gatekeeping here. 💅

vecscreen searches a query for segments that match any sequence in a specialized non-redundant vector database (UniVec). the search uses BLAST with parameters preset for optimal detection of vector contamination. those segments of the query that match vector sequences are categorized according to the strength of the match, and their locations are displayed (see example positive result). it's giving "here's exactly where the sus parts are" energy ✨

vecscreen is designed to quickly check a nucleic acid sequence for the presence of vector contamination and to show which segments within the sequence may be of vector origin. ngl, although a vecscreen search against UniVec will not identify the vector that is the most likely source of the contamination, this can usually be deduced from the cloning history of the sequenced DNA. so like, it points at the vibe, you figure out the name 😭

🎀 OPTIONS 🎀

a summary of options is included below. yes, all of them, we're not skipping a single one 👑

• - — Print usage message. a lil cry for help, but make it helpful 🫶
• -d str — Database (default = UniVec) 📚
• -f N — Output format:
  • 0 — HTML format, with alignments (default)
  • 1 — HTML format, no alignments
  • 2 — Text list, with alignments
  • 3 — Text list, no alignments
• -i filename — Query File (default = stdin) 🧚
• -o filename — VecScreen report Output File (default = stdout) 💜

👑 AUTHOR 👑

The National Center for Biotechnology Information. iconic. no cap.

🌈 SEE ALSO 🌈

<http://www.ncbi.nlm.nih.gov/VecScreen/VecScreen_docs.html>

and that's the whole page bestie, you ate it up fr fr 🍓

this little gem comes to you from NCBI, dated 2001-10-05, and it lives in section VECSCREEN(1) of the manual. main character energy since 2001, periodt 💅✨