POLISH_CLUSTERS(1)

πŸ’– POLISH_CLUSTERS πŸ’–

okay bestie, welcome to the polish_clusters(1) manual page β€” yes that's the section 1, user commands, we're in the big leagues now πŸ‘‘


✨ NAME ✨

polish_clusters β€” a whole lil collection of tools that annotate genomes using long read transcriptomics data πŸ§¬πŸ¦‹


🌸 DESCRIPTION 🌸

πŸŽ€ Usage of polish_clusters πŸŽ€

so like, here's the vibe β€” this is how you call it, and here is every single flag it takes. no notes, no skips, we're doing all of them πŸ’…

  • -V β€” prints out the version. quick lil flex, tells you what you're running ✨
  • -a string β€” read cluster memberships in tabular format. that's the input, bestie, hand it over 🫢
  • -c int β€” minimum cluster size. (default 1) tiny clusters need not apply πŸ™ˆ
  • -d string β€” location of the temporary directory. where the chaos happens behind the scenes πŸŒ€
  • -h β€” print out the help message. the classic "wait what do I do" button 😭
  • -m β€” do not load all reads in memory (slower). yes it's slower, no we're not sugarcoating it, it's literally in the docs β€” it trades speed for RAM and that's the whole deal πŸ’€
  • -o string β€” output fasta file. your final product, the main character output πŸ‘‘
  • -t int β€” number of cores to use. (default 4) go ahead and give it more, she's hungry πŸ”₯
  • -x string β€” arguments passed to minimap2. whatever you put here gets handed straight over, no middleman 🌈
  • -y string β€” arguments passed to racon. same deal, straight through, exactly as you wrote it ⭐
ngl, -x and -y are just lil relay batons β€” polish_clusters doesn't interpret them, it just passes them along, so whatever those tools accept is on you πŸŽ€

πŸ’œ AUTHOR πŸ’œ

this manpage was written by Nilesh Patra for the Debian distribution, and it can be used for any other usage of the program. iconic behaviour, we love a flexible license moment 🫢✨


and that's the whole page, bestie πŸ’– this was polish_clusters version 0.1.0+ds, dated September 2020 β€” go annotate some genomes, you've got this πŸŒΈπŸ¦‹πŸ’