ncbi-blast+(1)

✨ NAME ✨

ncbi-blast+ is the NCBI command tools for BLAST+ 💖 Manual: ncbi-blast+(1), in the USER COMMANDS section 🎀

📚 ONELINE DOCUMENTATION 📚

okay so the full BLAST+ docs live online at http://www.ncbi.nlm.nih.gov/books/NBK1763/ 🌸 bookmark that bestie 🔖

🧠 DESCRIPTION 🧠

okay so the NCBI BLAST+ is a group of tools for basic alignment search 🔍 and it's a rewrite of BLAST with new features and performance improvements ✨ the way it just glows up 😭

BLAST+ commands are different from the original BLAST, so if you're a BLAST user, you need to read the BLAST USERS paragraph first 🫶 no skipping it, periodt 💅

⚙️ CONFIGURATION ⚙️

BLAST+ uses the BLASTDB environment variable to find the default db directory 🗂️

you can also update the file /etc/ncbi/.ncbirc to add the following 🎀

[BLAST] BLASTDB=/var/lib/ncbi-blast+/db

🦋 BLAST USERS 🦋

the easiest way to get started with these command line apps is the legacy_blast.pl PERL script 💜 it's bundled with the BLAST+ applications and it's literally so helpful 😭

to use it, just prefix it to the invocation of the C toolkit BLAST command line application 💅 then append the --path option pointing to the installation directory of the BLAST+ applications ✨

for example, instead of using this 🙈

blastall -i query -d nr -o blast.out

you use this instead 🌈

legacy_blast.pl blastall -i query -d nr -o blast.out --path /usr/bin

👑 AUTHOR 👑

Olivier Sallou (olivier.sallou (at) irisa.fr) wrote the man page and packaging 🧚, and the NCBI - BLAST+ team made the tools 🔥

bestie, this page is version 2.2.25 from April 29, 2011 🫶💖 stay curious and keep aligning ✨🦋