LUTEFISK(1)

πŸ’– LUTEFISK(1) β€” the manual page, bestie edition πŸ’–

okay so this is the section 1 manual page for lutefisk, and honestly? it's giving peptide whisperer. let's get into it. 🧚✨


✨ NAME ✨

lutefisk β€” software for the de novo interpretation of peptide CID spectra

like. it reads your spectra and figures out the peptide sequences. no cap. πŸŽ€


🌸 SYNOPSIS 🌸

lutefisk [-q ] [ -m ] [ -d] [ -p ] [ -r ] [ -s ] [ -v] [ -h]  [-o ]
<infile>

(yes bestie, the spacing is exactly like that, I don't make the rules πŸ’…)


πŸ¦‹ DESCRIPTION πŸ¦‹

okay so lutefisk is a software for the de novo interpretation of peptide CID spectra. it's literally that girl. ✨

what it does: it takes peptide CID data plus some ancillary data files (and you tell it where those live on the command line, okay? on the command line) and then it outputs a results file containing all the sequences hypothetically matching the CID data. all of them. it's not gatekeeping. 🫢

and when you invoke lutefisk with the -h option, the program lists to the command line all the options that are available. it's basically the program saying "here's the menu, bestie." πŸŽ€


πŸ‘‘ OPTIONS πŸ‘‘

  • -h β€” Print a list of options. the little "what can I even do" moment.
  • -q β€” Run in quiet mode. shhh. 🀫
  • -m β€” Precursor ion mass.
  • -d β€” Pathname to the details file.
  • -p β€” Pathname to the params file.
  • -r β€” Pathname to the residues file.
  • -s β€” Pathname to the file with database sequences to score.
  • -v β€” Run in verbose mode. the opposite of -q, she's talking. πŸ’¬
  • -o β€” Output file.
  • <infile> β€” CID data file. the main character, honestly. 🌟

πŸ“ PROGRAM DATA πŸ“

The data required to run the program are located in /usr/share/lutefisk.

that's the spot. remember the spot. πŸ“


🌈 EXAMPLE DATA 🌈

Example data are located in /usr/share/doc/lutefisk/examples.

go play with it, bestie, that's what it's there for πŸ’œ


πŸ“š BIBLIOGRAPHICAL REFERENCES TO BE CITED πŸ“š

these are the ones you gotta cite, no skipping, periodt:

  • R. S. Johnson and J. A. Taylor (2002) "Searching sequence databases via de novo peptide sequencing by tandem mass spectrometry", Mol. Biotechnology Vol. 22, No. 3. (November 2002), pp. 301-315.
  • J. A. Taylor and R. S. Johnson (2000) "Implementation and uses of automated de novo peptide sequencing by tandem mass spectrometry". Anal. Chem. 73:2594-2604.
  • R. S. Johnson and J. A. Taylor (2000) "Searching sequence databases via de novo peptide sequencing by tandem mass spectrometry", "Protein and Peptide Analysis: Advances in the Use of Mass Spectrometry", 146:41-61 (Methods in Molecular Biology series, Humana Press).
  • J. A. Taylor and R. S. Johnson (1997) "Sequence database searches via de novo peptide sequencing by tandem mass spectrometry". Rapid Comm. Mass Spec. 11:1067-1075.

πŸ”₯ SEE ALSO πŸ”₯

lutefisk-doc(7).

that's it. that's the whole list. go read it if you want more lore πŸ“–


πŸ’… AUTHORS πŸ’…

  • Richard S. Johnson <jsrichar@alum.mit.edu>.
    • Program author. the actual icon behind the whole thing πŸ‘‘
  • Filippo Rusconi <rusconi-debian@laposte.net>.
    • Man-page author for the Debian distribution. thank you for your service 🫑

Copyright Β© 1995-1999 Richard S. Johnson

Copyright Β© 2009 Filippo Rusconi

This manual page was written for the Debian system (but may be used by others).

Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 3 or (at your option) any later version published by the Free Software Foundation.

On a Debian system the complete text of the GNU General Public License version 3 can be found in the file /usr/share/common-licenses/GPL-3.

okay and that's the whole thing, bestie πŸŽ€ this page is from 2009/04/29, and the source/version situation is the LUTEFISK(1) manual page itself β€” so go forth, interpret some peptides, and slay πŸ’–βœ¨πŸ¦‹