KMC_TOOLS-KMERS_SUBTRACT(1)

πŸ’– KMC_TOOLS-KMERS_SUBTRACT πŸ’–

okay bestie, welcome to the kmc_tools-kmers_subtract page, straight outta the KMC_TOOLS-KMERS_SUBTRACT(1) manual πŸ’… this is the one that does subtraction, and she eats every time πŸ”₯


✨ NAME ✨

kmc_tools-kmers_subtract β€” calculate subtraction of two KMC databases πŸ¦‹


🌸 SYNOPSIS 🌸

kmc_tools kmers_subtract <input1 [input1_params]> <input2
[input2_params]> <output [output_params]>

πŸŽ€ DESCRIPTION πŸŽ€

so basically?? the output database will contains only k-mers that are present in the first input set but absent in the second one πŸ‘‘ and the counter value is equal to the value from the first input set, no cap πŸ’―


✨ OPTIONS ✨

For each input there are additional parameters, bestie, don't skip these:

  • -ci<value> β€” exclude k-mers occurring less than <value> times πŸ™ˆ
  • -cx<value> β€” exclude k-mers occurring more of than <value> times πŸ’€

πŸ’œ For output there are additional parameters πŸ’œ

  • -ci<value> β€” exclude k-mers occurring less than <value> times 🌸
  • -cx<value> β€” exclude k-mers occurring more of than <value> times 🫢
  • -cs<value> β€” maximal value of a counter ⭐

πŸ¦‹ EXAMPLE πŸ¦‹

look at her go, literally iconic:

kmc -k28 file1.fastq kmers1 tmp
kmc -k28 file2.fastq kmers2 tmp
kmc_tools kmers_subtract kmers1 kmers2 kmers1_kmers2_subtract -cs200

πŸ‘‘ AUTHOR πŸ‘‘

KMC was originally written by, and we stan:

  • Sebastian Deorowicz (sebastian.deorowicz@polsl.pl) πŸ’–
  • Marek Kokot ✨
  • Szymon Grabowski 🌈
  • Agnieszka Debudaj-Grabysz πŸŽ€

πŸ’… COPYING πŸ’…

KMC is a free software distributed under GNU GPL3 licence for academic, research, and commercial use 🫢


and that's the whole vibe, bestie πŸ’– this was KMC_TOOLS-KMERS_SUBTRACT(1), the very last line of the page, no date or version dropped alongside it β€” just the page name itself, so that's what we're signing off with 🌟 slay, go subtract some k-mers πŸ§šπŸ’œ

πŸ“¦ where this page comes from