KMC_TOOLS-COUNTERS_SUBTRACT(1)

✨ KMC_TOOLS-COUNTERS_SUBTRACT(1) ✨

okay bestie, so this is a page from the kmc_tools manual, section 1 (general commands, iykyk). sit down, grab a boba, we're doing counter subtraction 💅

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🌸 NAME 🌸

kmc_tools-counters_subtract — the way that it calculates the counters subtraction of two KMC databases 🧮✨

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🦋 SYNOPSIS 🦋

kmc_tools counters_subtract <input1 [input1_params]> <input2 [input2_params]> <output [output_params]>

two inputs, one output, and optional lil params hanging off each one. that's the whole vibe 🎀

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💖 DESCRIPTION 💖

so here's the tea 🍓 the output database is gonna contain only the k-mers that are present in the first input set and that have counters higher than the appropriate k-mers in the second set. like, if it's not winning, it's not invited bestie 💀

and for each k-mer that makes the cut, the counter equals the difference between the counter in the first set and the counter in the second set. literally subtraction. math is mathing ✨

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👑 OPTIONS 👑

💜 For each input there are additional parameters:

• -ci<value> — exclude k-mers occurring less than <value> times 🙅‍♀️
• -cx<value> — exclude k-mers occurring more of than <value> times 🙅‍♀️

🌈 For output there are additional parameters:

• -ci<value> — exclude k-mers occurring less than <value> times 🙅‍♀️
• -cx<value> — exclude k-mers occurring more of than <value> times 🙅‍♀️
• -cs<value> — maximal value of a counter, so it doesn't get too main character 📈

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🧚 EXAMPLE 🧚

watch me cook 🍳

kmc - k28 file1.fastq kmers1 tmp
kmc - k28 file2.fastq kmers2 tmp
kmc_tools counters_subtract kmers1 kmers2 kmers1_kmers2_counters_subtract

build two databases, then subtract them. ate, no crumbs 🔥

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⭐ AUTHOR ⭐

KMC was originally written by these absolute legends:

• Sebastian Deorowicz (sebastian.deorowicz@polsl.pl)
• Marek Kokot
• Szymon Grabowski
• Agnieszka Debudaj-Grabysz

not me being obsessed with a whole squad of iconique authors 👑

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💅 COPYING 💅

KMC is a free software distributed under GNU GPL3 licence for academic, research, and commercial use. slay, honestly 🫶

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okay that's the whole page bestie, go forth and subtract those counters, the output db is not gonna build itself 😭💖

KMC_TOOLS-COUNTERS_SUBTRACT(1) — that's the footer babes, and it's giving "no extra version or date info on this one", so that's all she wrote 🌸 bye pookie 💜