INFO_ON_SEQS(1)

📦
version 6.2.1 · 4 versions
, in ubuntu stonking

✨ info_on_seqs — the manual, but make it make sense ✨

okay bestie, buckle up 💖 this is info_on_seqs from the User Commands girlies, and she's the final boss step of SIFT prediction. lowkey important, highkey under-documented. let's get into it 🫶

🌸 NAME 🌸

info_on_seqs — bestie, this does the final step of SIFT prediction. that's it, that's the vibe. ✨

🎀 SYNOPSIS 🎀

info_on_seqs <FASTA_FILE> <SUBSTITUTIONS_FILE> <OUTPUT>

three arguments, no options, no flags, no drama. she's simple like that 💅

🦋 DESCRIPTION 🦋

okay so real talk, the actual truth first: you should be using SIFT_for_submitting_fasta_seq.csh instead of this program, unless you genuinely know what you're doing. not me warning you twice, but like… i am. 😭

this program prepares the final SIFT prediction. and here's the plot twist — in addition to the command line arguments, it expects certain files to be present next to the FASTA_FILE. so like, it's looking around, it's checking its surroundings, it needs its little friends nearby. don't ghost it. 🙈

💅 OPTIONS 💅

  • <FASTA_FILE> — protein sequence in fasta format. the main character of the whole operation, fr fr. 🧚
  • <SUBSTITUTIONS_FILE> — the file of substitutions to be predicted. not sure about the format? go peek at /usr/share/doc/sift/examples/lacI.subst for an example, it's right there. and here's the spicy bit: if you pass - as this argument, then scores for all mutations of the entire protein sequence are printed. yes, the whole thing. every last one. 🔥
  • <OUTPUT> — output file. where the receipts go. 💖

👑 AUTHOR 👑

Pauline Ng — iconic, she did that.

(C) Copyright 1993-2001, Fred Hutchinson Cancer Research Center

noncommercial license, bestie. check /usr/share/doc/sift/copyright for the full details before you go doing anything wild. 💀

🌈 SEE ALSO 🌈

  • SIFT_for_submitting_fasta_seq.csh — literally the one you should probably be using. yes i'm bringing it up again. iykyk. ✨

and that's the whole page, you ate it up 🎀 this was version 6.2.1, dated 2026-10-09, and yeah it's filed under INFO_ON_SEQS(1) — go be the main character of your own prediction pipeline, bestie 💖🦋✨

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