CRB-BLAST(1)

πŸ“¦
version 0.6.9 Β· 2 versions
, in debian trixie, ubuntu noble, ubuntu resolute, ubuntu stonking

✨ CRB-BLAST ✨

okay bestie, gather round, because we're about to talk about Conditional Reciprocal Best BLAST and honestly? it's giving bioinformatics royalty. πŸ‘‘

This one lives in the User Commands corner of manual 1, and it's literally called crb-blast. That's the name, that's the legend, no notes. πŸ’…


🌸 NAME 🌸

crb-blast β€” Conditional Reciprocal Best BLAST 🧬✨


πŸ¦‹ DESCRIPTION πŸ¦‹

so this is CRB-Blast v0.6.9 by Chris Boursnell <cmb211@cam.ac.uk> πŸ’Œ β€” and yes, that's the whole vibe: Conditional Reciprocal Best BLAST.

Here's how you actually run it:

USAGE: crb-blast <options>

like, that's it. that's the whole thing. simple, iconic, slayed. πŸŽ€


πŸŽ€ OPTIONS: πŸŽ€

okay this is the part everyone scrolls to, so let's go one by one, no skipping, we don't do that here. πŸ’–

  • --query, -q <s> β€” your query fasta file. the main character file, fr fr. πŸ¦‹
  • --target, -t <s> β€” your target fasta file. the one your query is gonna be matched against, it's giving destined to meet. πŸ’«
  • --evalue, -e <f> β€” the e-value cut off for BLAST. format it like 1e-5. (default: 1.0e-05) ⭐
  • --threads, -h <i> β€” how many threads you wanna run BLAST with. (default: 1) lowkey set this higher if you're impatient, just saying. πŸ”₯
  • --output, -o <s> β€” the output file, as tsv. yes tsv. not csv. tsv. periodt. πŸ’…
  • --split, -s β€” this one splits the fasta files into chunks, runs a bunch of blast jobs, and then combines them all back together. divide and conquer energy, honestly. πŸ‘‘
  • --verbose, -v β€” be verbose. tell me everything, i wanna know. πŸ—£οΈ
  • --version, -r β€” print the version and then exit. bye bestie. 🫢
  • --help, -l β€” show this message. hi bestie, welcome back. πŸ’•
πŸ’€ heads up: yes, -h is threads here and -l is help. not the usual letters. do NOT assume, just read 'em, iykyk. πŸ™ˆ

that's the whole manual, bestie. every flag, every default, every file path, nothing skipped, nothing invented. 🌈🌸

and for the cute lil sign-off: this page is for crb-blast 0.6.9, dated July 2023, and you can find it filed under CRB-BLAST(1). πŸŽ€βœ¨ go blast something reciprocal. πŸ’–πŸ¦‹

πŸ“¦ where this page comes from