BARRNAP(1)

📦
version 1.0.0 · 2 versions
, in ubuntu stonking

🌸 BARRNAP(1) 🌸

it's the barrnap user manual bestie — part of the User Commands squad 💖

✨ NAME ✨

barrnap — okay so this is the girl who predicts where ribosomal RNA genes are hiding in your genomes 🔍🧬 fr fr

💅 DESCRIPTION 💅

📖 Synopsis:

barrnap 0.9 - rapid ribosomal RNA prediction
lowkey iconic, highkey fast ⚡

👑 Author:

Torsten Seemann — the main character who made this happen 🫶

🛠️ Usage:

barrnap [options] chr.fa
barrnap [options] < chr.fa
barrnap [options] - < chr.fa

so like, three ways to feed her: a file, a redirect, or a - with a redirect. pick your vibe, she eats all of them 🍓

🌈 OPTIONS 🌈

  • --help — prints this help. the "wait what does this do again" bestie 💖
  • --version — shows the version and then immediately exits. she's done, she's leaving, periodt 💀
  • --citation — prints the citation so you can reference barrnap properly. cite your sources, that's the rule ✨
  • --kingdom [X] — which kingdom are we doing?? your options are arc, bac, euk, mito. default is 'bac' 👑
  • --quiet — no screen output at all. default is OFF. she's SILENT, not a word, not a peep 🙈
  • --threads [N] — how many threads/cores/CPUs you wanna throw at her. default is '1' — give her more, she'll slay faster 🔥
  • --lencutoff [n.n] — a proportional length threshold, and if a hit is under it she labels it as partial. default is '0.8' 🎀
  • --reject [n.n] — proportional length threshold where she straight up rejects the prediction. default is '0.25' 💀
  • --evalue [n.n] — the similarity e-value cut-off. default is '1e-06' 🧚
  • --incseq — include FASTA input sequences in the GFF3 output. default is OFF. not on by default bestie, you gotta ask 🌸
  • --outseq [X] — save the rRNA hit seqs into this FASTA file. default is '' (aka nowhere, aka empty) 🦋
⚠️ heads up: the one-liners above are all the original gives you — no secret extra defaults hiding in here, iykyk. if a default says OFF or '', that is genuinely the default, no cap.

and that's the whole page, bestie!! this was barrnap version 0.9 from March 2026, and yeah she's filed under BARRNAP(1) 🎀✨💜

go predict some rRNA genes, you absolute legend 🌈

📦 where this page comes from