BARCODE_SPLIT(1)

🎀 BARCODE_SPLIT(1) · User Commands 🎀

✨ NAME ✨

okay so Barcode_split is a simple tool that splits a sequencing summary report into per-barcode files 💖 It's giving organized energy.

📜 DESCRIPTION 📜

Here's the usage line, bestie 💅

usage: Barcode_split [-h] [--version] --summary_file [SUMMARY_FILE ...]

[--barcode_file [BARCODE_FILE ...]]
        [--output_dir OUTPUT_DIR] [--output_unclassified]
        [--min_barcode_percent MIN_BARCODE_PERCENT] [-v | -q]

Barcode_split is a simple tool to split sequencing summary report in per barcodes. Literally the whole vibe ✨

🌸 optional arguments 🌸

• -h, --help — shows the help message and exits. Main character energy 👑
• --version — shows the program's version number and exits 🦋
• --summary_file [SUMMARY_FILE ...], -f [SUMMARY_FILE ...] — the path to a sequencing_summary made by Albacore 1.0.0 + (read_fast5_basecaller.py) or Guppy 2.1.3+ (guppy_basecaller). You can also pass multiple space separated file paths, or a UNIX style regex that matches multiple files 🧚
• --barcode_file [BARCODE_FILE ...], -b [BARCODE_FILE ...] — the path to the barcode_file made by Guppy 2.1.3+ (guppy_barcoder) or Deepbinner 0.2.0+. Same deal: multiple space separated paths or a UNIX style regex works too 🍓
• --output_dir OUTPUT_DIR, -o OUTPUT_DIR — the folder where your split barcode data gets dumped. If you don't pick one, it defaults to the current dir 📂
• --output_unclassified, -u — if you add this, unclassified barcodes get written to a file too. By default they get skipped, so no drama 🙈
• --min_barcode_percent MIN_BARCODE_PERCENT, -p MIN_BARCODE_PERCENT — the minimum percent of total reads a barcode needs to keep its label. If it falls below that, the barcode value gets set to unclassified 💀 (default: 0.1)
• -v, --verbose — turns verbosity up. More chatter, more vibes 🔊
• -q, --quiet — turns verbosity down. Shh, we're focused 🤫

💜 AUTHOR 💜

This manpage was written by Nilesh Patra for the Debian distribution, and it's free to use for any other usage of the program 🫶

Barcode_split 2.5.2 · December 2020 · BARCODE_SPLIT(1) 🌈✨ Stay iconic, bestie 💖