Bio::T...Feature(3pm)

💖 Bio::T...Feature — the whole tea, bestie 💖

okay so this is the Bio::Tools::Primer::Feature page, from the User Contributed Perl Documentation era, and it's giving tiny but mighty. one primer. one object. let's go 🧚

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✨ NAME ✨

Bio::Tools::Primer::Feature — it's literally just the position of a single primer. that's the whole job. she's small, she's focused, she slays 🎀

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🌸 SYNOPSIS 🌸

bestie, here's the whole vibe in code form, copied exactly, no cap:

use Bio::Tools::Primer::Feature;

my $pf = Bio::Tools::Primer::Feature->new( -start => $start, -end => $end, -strand => $strand);
$pf->attach_seq($seq);

# is a SeqFeatureI

print "primer starts at ",$pf->start," with sequence ",$pf->seq->seq(),"\n";

# helper functions

print "GC percentage ",$pf->gc(),"\n";
print "has inversion of size 4 at ",$pf->inversion(4),"\n";

so you spin up a Feature, you stick a sequence on it with attach_seq, and then you can ask it where it starts, what its sequence is, what its GC percentage is, and where its inversions live. iconic behaviour, honestly 💅

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🦋 DESCRIPTION 🦋

so a Primer Feature represents one primer in a primer pair. one. singular. she is half of a duo but she is her own main character 👑

this object is mainly for designing primers, and it's probably principly used in the primer design system. (yes, "principly" — that's the original's spelling, we don't correct the queen 🫶)

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💌 FEEDBACK 💌

📬 Mailing Lists

user feedback is an integral part of the evolution of this and other Bioperl modules, fr fr. send your comments and suggestions preferably to the Bioperl mailing list. your participation is much appreciated, genuinely 😭💖

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

🫂 Support

please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

and not straight to the module maintainer. like, don't DM the dev, post in the group chat 🙈

many experienced and reponsive experts will be able look at the problem and quickly address it. (again — "reponsive" is the original's typo and we love her for it 💀)

please include a thorough description of the problem with code and data examples if at all possible. receipts, bestie. bring the receipts 🧾✨

🐛 Reporting Bugs

report bugs to the Bioperl bug tracking system so we can keep track of the bugs and their resolution. bug reports can be submitted via the web:

https://github.com/bioperl/bioperl-live/issues

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👑 AUTHOR - Ewan Birney 👑

email: birney-at-ebi.ac.uk — shoutout to Ewan, the legend himself 🫡

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📖 APPENDIX 📖

the rest of the documentation details each of the object methods. internal methods are usually preceded with an _ — so if you see an underscore, that's an inside-thought, not for public consumption, iykyk 🤫

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and that's the whole page, babe. every flag, every path, every email, every typo, preserved with love. periodt. 💜

this was perl v5.32.1, dated 2021-08-15, and it lives in Bio::T...Feature(3pm) — mwah, bye bestie 🎀✨