WIGEON(1)

✨ WIGEON ✨

okay bestie so the manual page calls this thing wigeon, and honestly? it's the reimplementation of the Pintail 16S DNA anomaly detection utility. yes that's the whole name. yes it's a mouthful. we love her anyway 🦋

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🌸 DESCRIPTION 🌸

so here's the vibe: WigeoN looks at the sequence conservation between a query and a trusted reference sequence, and both of those have to be in NAST alignment format. based on how identical the query and the reference are, you'd expect a certain amount of variation to show up in the alignment, right? well — if the variation you actually observe is bigger than the 95% quantile of the distribution of variation seen between non-anomalous sequences, boom, it gets flagged as an anomaly. periodt 💅

WigeoN is a flexible command-line based reimplementation of the Pintail algorithm — citation drop, don't skip it: Appl Environ Microbiol. 2005 Dec;7112:7724-36.

💀 real talk though: WigeoN is only useful for flagging chimeras and anomalies in near full-length 16S rRNA sequences. it lowkey lacks sensitivity with sequences under 1000 bp. she said what she said.

to actually run WigeoN you need NAST-formatted sequences, and you get those from the nast-ier utility. no shortcuts bestie 🙈

also she's part of the microbiomeutil suite 🫶

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🎀 OPTIONS 🎀

👑 Required:

• --query_NAST — multi-fasta file containing query sequences in alignment format. this one is non-negotiable, you literally cannot run without it ✨

🦋 Optional:

• --db_NAST — db in NAST format
• --db_FASTA — db in fasta format (megablast formatted)
• --num_top_hits — default 1: uses only the single best match. main character behavior honestly, only the best one gets picked 👑
• --plot
• --DEBUG
• --exec_dir — cd to exec_dir before running

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🌈 SEE ALSO 🌈

http://microbiomeutil.sourceforge.net/#A_WigeoN — go click it, iykyk 💖

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💜 AUTHOR 💜

this manual page was written by Andreas Tille <tille@debian.org> — and ngl the icon himself said it can be freely used for any other distribution 🫶 slay.

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and that's the whole thing!! this page is from the wigeon manual, dated June 2013 🌸 mwah, go flag some anomalies bestie 💅✨