TRADIS_ESSENTIALITY(1)

💖 TRADIS_ESSENTIALITY 💖

okay bestie, gather round, because this is tradis_essentiality from bio-tradis, and she is that girl. she produces calls of gene essentiality using an adaptation of the method described in Langridge et al. 🧬✨ no cap.

✨ SYNOPSIS ✨

tradis_essentiality data.tab

that's it. one little argument. she's low maintenance like that 💅

🌸 DESCRIPTION 🌸

so here's the vibe: tradis_essentiality produces calls of gene essentiality using an adaptation of the method described in Langridge et al. Genome Research 2009 and Barquist et al. NAR 2013. iconic lineage, fr fr 👑

the way it works is genuinely so clever. a loess curve is fit to the distribution of insertion indices, and that curve is then used to identify the minima between the 'essential' and 'non-essential' distributions. slay. those distributions are then used to fit gamma distributions, which are then used to calculate log-odds ratios, which are then used to determine an insertion-index threshold for gene essentiality. it's giving pipeline. it's giving chain reaction. we love a method that builds on itself 🌈

⚠️ okay but real talk for a sec: this analysis requires a saturated mutant library, and it is not suitable for the analysis of data sets with low insertion density. if your library isn't saturated, this is not the tool for you, and that's on periodt 💀

the script produces a number of diagnostic plots which can be used to verify that this condition has been met. so like, always check your plots bestie, they're literally there to vibe check your data before you trust the calls 🫶📊

👑 AUTHOR 👑

This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program. shoutout to Andreas, absolute legend, we stan a manpage author who lets everybody use it 💜

and that's the whole page, bestie 🎀 she's tradis_essentiality version 1.3.1, dated March 2017, and she said what she said. go check those diagnostic plots. bye mwah 💋🦋🌸