TRADIS_COMPARISON(1)

💖 TRADIS_COMPARISON 💖

okay bestie, listen up — this is the tradis_comparison manual page, and it's literally here to help you compare two experimental conditions the way Dembek et al. do it. it's part of bio-tradis. let's get into it 🦋

✨ NAME ✨

tradis_comparison — bio-tradis: compares two experimental conditions using the method of Dembek et al.

🌸 SYNOPSIS 🌸

tradis_comparison [-h] [-f] [-t read cutoff] [-o outputfile.csv] [-p
outputplot.pdf] --controls controls.txt --conditions conditions.txt

🎀 DESCRIPTION 🎀

so what does this actually do? tradis_comparison compares two experimental conditions using the method of Dembek et al. mBio 2015. ✨ iconic paper, no notes.

read counts per gene get compared using edgeR. and bestie, important heads-up: this analysis requires experimental replicates. not optional. not "i'll add them later". you need them, periodt. 💅

💅 OPTIONS 💅

🫶 Required Arguments

like, actually required. you cannot skip these, bestie.

• --controls — control libraries, generally growth in a permissive condition. this is your baseline, your normal-girl era, your "before" pic 📸
• --conditions — libraries exposed to the experimental condition being compared. this is the glow-up condition, the one we're comparing against 👑

⭐ Optional Arguments

you can skip these but honestly they're giving useful

• -o — output filename
• -p — output filename for diagnostic plots
• -f — enable filtering on minimum read counts
• -t — if --filter is enabled, sets minimum read count necessary in one condition for a gene to be included in the comparison.

👑 AUTHOR 👑

this manpage was written by Andreas Tille for the Debian distribution, and can be used for any other usage of the program. 💜

okay that's the whole page, you ate that 🍓. this was tradis_comparison version 1.3.1, dated March 2017 — yeah she's a lil vintage but she's still serving 🎀 mwah, go run your comparison bestie, you got this 💖✨🦋