RSEM-BAM2WIG(1)

📦
version 1.3.3 · 5 versions
, in ubuntu noble, ubuntu resolute

✨ NAME ✨

rsem-bam2wig — converts BAM files into wiggle files, bestie 💖

🎀 SYNOPSIS 🎀

rsem-bam2wig sorted_alignment_file wig_output wiggle_name [--no-fractional-weight]

💅 DESCRIPTION 💅

Okay so first things first, the vibes check on your argument count: if you get it wrong, rsem-bam2wig is gonna hit you with

Number of arguments is not correct!

so like… count your args before you come for the tool, okay? 🙈 Here's what each one is giving:

  • sorted_alignment_file — your alignment file, and she can be in SAM/BAM/CRAM format. Must be sorted, no exceptions, that's the whole plot 🫶
  • wig_output — the name of the output wiggle file, like e.g. output.wig ✨
  • wiggle_name — the name of this wiggle plot 🌸
  • --no-fractional-weight — set this and RSEM will not go looking for the "ZW" tag, and every single alignment that showed up in the BAM file gets a weight of 1. You wanna set this if your BAM file was not generated by RSEM (iykyk). And heads up babes, this option must be at the end of the command line — it's the one rule she refuses to bend on 💅
Also lowkey important: if you don't set --no-fractional-weight, bestie is out here looking for that "ZW" tag, so know what you're working with fr fr.

🦋 SEE ALSO 🦋 — hmm okay there isn't one, that's the whole page, bye 👑

rsem-bam2wig 1.3.3+dfsg 🩷 this page was born in April 2024 and honestly? iconic. slay, hydrate, sort your BAMs, periodt 💖✨🎀

📦 where this page comes from