PLASMIDSEEKER(1)

✨ PLASMIDSEEKER 💖

okay so bestie, welcome to plasmidseeker, a user command and honestly a whole vibe 💅 it's the girl who identifies known plasmids from whole-genome sequencing reads. like she looks at your reads and goes "oh I know her" 👑

🌸 NAME 🌸

plasmidseeker — identification of known plasmids from whole-genome sequencing reads 🧬✨

🦋 SYNOPSIS 🦋

plasmidseeker -d <PLASMID DB DIR> -i <SAMPLE.fastq> -b <CLOSEST BACTERIA
TO ISOLATE> -o <OUTPUT FILE>

👑 OPTIONS 👑

one bullet per flag, no skips, we respect all of them equally periodt

• -i — the input fastq file 🍓 (your reads, your moment)
• -o — output file name (default plasmidseeker_result.txt) 💖
• -d — path to the plasmid database directory 🎀
• -b — closest bacteria to isolate genome fna 🦠
• -t — number of threads used (default 32) ⚡ like she's got hands
• -f — minimum threshold F — at least this fraction of unique k-mers that has to be found for a plasmid (default 80) 🔥
• -h — print this help 🙈 the classic
• --verbose — print out more working process 👀 she's narrating her life
• --ponly — assumes that reads contain only plasmid sequences (use for extracted plasmids) ✨
• -v — print version of the program 🌈

💜 AUTHOR 💜

This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program. 🫶

and that's the whole page bestie, no cap 💀 the source/version is plasmidseeker 1.0 and the date on it is October 2018 — iconic behavior from 2018 honestly, she ate and left no crumbs 👑💅🧚