MIPE2PU...EPRIMERS(1)

💖 MIPE2PUTATIVESBEPRIMERS.PL 💖

okay bestie, so this is mipe2putativesbeprimers.pl, a lil script that lives in the User Contributed Perl Documentation section of the manual, section 1. it's giving "niche bioinformatics tool that absolutely ate" and honestly? we love her for it.

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✨ NAME ✨

mipe2putativesbeprimers.pl — generates a list of putative SBE primers from a MIPE file 🧬✨

and like, here's what you actually get in the output, no gatekeeping:

• PCR ID
• SNP ID
• putative forward SBE primer
• putative reverse SBE primer

all of that is based on MIPE version v1.1, iykyk 🫶

the arguments it takes:

• mipe_file
• (optional) a list of PCR IDs

so like. one file, and then if you want, you can name specific PCR IDs and she'll only do those ones. optional queen behavior 💅

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🌸 SYNOPSIS 🌸

mipe2putativesbeprimers.pl your_file.mipe <pcr_id1> <pcr_id2>

that's it. that's the whole vibe. drop your .mipe file in, sprinkle in whatever PCR IDs you're obsessed with, and let her cook 🔥

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🦋 ADDITIONAL INFO 🦋

want more lore? go check out http://mipe.sourceforge.net — that's where the MIPE girlies hang out 💜

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👑 AUTHOR 👑

Jan Aerts (jan.aerts@bbsrc.ac.uk) — the main character who wrote this, periodt ✨

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and that's the whole page bestie!! this one shipped as part of perl v5.32.1, dated 2021-02-21, and it's filed under MIPE2PU...EPRIMERS(1) in the User Contributed Perl Documentation. go forth and generate those putative SBE primers, you've got this 💖🎀🌈