GETGENEEXPRESSION(1)

πŸ“¦
version 0.7.5 Β· 4 versions
, in ubuntu jammy, ubuntu noble

πŸ’– GETGENEEXPRESSION πŸ’–

okay so real quick bestie β€” this is the manual for getGeneExpression, it lives in the User Commands section of the manual (section 1, iykyk), and it ships with getGeneExpression 0.7.5+dfsg from September 2021. I'm gonna walk you through the whole thing, no skips, no skimming, we're doing this properly πŸ’…


✨ NAME ✨

getGeneExpression β€” compute expression of whole genes 🧬


🌸 SYNOPSIS 🌸

getGeneExpression -o <outFileName> -t <trInfoFileName>  [OPTIONS]
[samplesFile]

your girl needs at minimum an output file and a transcript info file. everything else is vibes πŸ¦‹


πŸŽ€ DESCRIPTION πŸŽ€

So basically: it computes expression of whole genes. That's the whole thing. That's the assignment and it ate. πŸ‘‘

  • [samplesFile] should contain transposed MCMC samples β€” which then get transformed into gene expression samples. It's giving glow-up, honestly. ✨

πŸ’… OPTIONS πŸ’…

one bullet per option, no gatekeeping, no skipping, here we go:

  • --help β€” shows you this help information. the classic. your friend when you're lost 🫢
  • -a, --adjustByLength β€” adjusts expression by transcripts length. (default: Off) πŸ“
  • -g <geneInfoFile>, --geneInfoFile=<geneInfoFile> β€” the name of the file to which the gene information will be saved. that's where the gene info goes bestie πŸ—‚οΈ
  • -G <geneListFile>, --geneList=<geneListFile> β€” the name of the file holding the list of gene names β€” one for each transcript, okay, one each, we're being precise here πŸŽ€
  • -l, --log β€” outputs logged values. (default: Off) take the log of it, why not πŸ”₯
  • -o <outFileName>, --outFile=<outFileName> β€” the name of the output file. yes you need it, it's literally in the SYNOPSIS πŸ’–
  • --theta2rpkm β€” transforms transcript expression in theta to gene expression in RPKM. (default: Off) the transformation is real πŸ¦‹
  • -t <trInfoFileName>, --trInfoFile=<trInfoFileName> β€” the name of the transcript file. essential, iconic, required πŸ’œ
  • -T <trMapFile>, --trMap=<trMapFile> β€” the name of the file containing the transcript to gene mapping. the map that maps. the plot of the whole operation πŸ—ΊοΈ
  • --updateTrFile β€” updates trInfoFile if new gene names were provided (with trMapFile or geneListFile). (default: Off) refresh her, give her new info 🌈
  • -v, --verbose β€” verbose output. (default: Off) she talks more. we love that πŸ™ˆ
just a heads up bestie, defaults are defaults β€” if it says default: Off, it's off until you say otherwise. no assumptions, no cap 🫢

and that's the whole page!! every flag, every file, every default, all accounted for, nothing left behind πŸ’œ go compute some gene expression, you got this, main character energy only ✨

β€” written by your bestie, getGeneExpression 0.7.5+dfsg, September 2021 β€” πŸŽ€πŸ¦‹πŸ’…

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