FASTAQ-TO_TILING_BAM(1)

💖 NAME 💖

fastaq_to_tiling_bam — bestie, this one makes a BAM file of reads that are spread uniformly across your input reference. perfect little tiling reads, no gaps, no drama ✨

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🌸 DESCRIPTION 🌸

okay so here's the vibe. the usage goes a lil something like this:

fastaq_to_tiling_bam [options] <infile> <read_length> <read_step> <read_prefix> <outfile>

You hand it a sequence file, and it hands you back a BAM file stuffed with perfect (unpaired) reads tiling the whole genome. the entire thing. every last base is covered, it's giving main character energy fr fr 👑

🎀 positional arguments (yes u have to give all of these) 🎀

• infile — the name of ur input fasta/q file. the raw material, the moment, the source 💅
• read_length — how long each read is. literally the length of reads.
• read_step — the distance between the start of each read. so like, how far apart they're vibing 🦋
• read_prefix — the prefix that goes on the read names. name ur children, bestie.
• outfile — the name of the output BAM file. where all the magic lands ✨

💅 options 💅

• -h, --help — show this help message and exit. classic. iconic. always there for u 🫶
• --qual_char QUAL_CHAR — the character to use for the quality score. default is I 🌸
• --read_group READ_GROUP — adds the given read group ID to all the reads. default is 42 😌

🚨 important (not me forgetting this): it assumes that samtools is in your path. so like, make sure it's there before u come for this program, k? no cap.

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🌈 SIGN OFF 🌈

this lil legend is fastaq_to_tiling_bam version 3.17.0, and it dropped in January 2026. periodt. go tile something gorgeous bestie 💖🧚⭐